Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.

@article{Tanimoto2000ContextdependentER,
  title={Context-dependent EKLF responsiveness defines the developmental specificity of the human epsilon-globin gene in erythroid cells of YAC transgenic mice.},
  author={Keiji Tanimoto and Qinghua Liu and Frank Grosveld and J{\"o}rg Bungert and James Douglas Engel},
  journal={Genes & development},
  year={2000},
  volume={14 21},
  pages={2778-94}
}
We explored the mechanism of definitive-stage epsilon-globin transcriptional inactivity within a human beta-globin YAC expressed in transgenic mice. We focused on the globin CAC and CAAT promoter motifs, as previous laboratory and clinical studies indicated a pivotal role for these elements in globin gene activation. A high-affinity CAC-binding site for the erythroid krüppel-like factor (EKLF) was placed in the epsilon-globin promoter at a position corresponding to that in the adult beta-globin… CONTINUE READING