• Chemistry
  • Published 2010

Content determination of the mid-profection of fat soluble vitamins (II) for Injection by RP-HPLC

@inproceedings{Xueyan2010ContentDO,
  title={Content determination of the mid-profection of fat soluble vitamins (II) for Injection by RP-HPLC},
  author={Chen Xue-yan},
  year={2010}
}
Objective:To establish a method for the content determination of the mid-profection of fat soluble vitamins (Ⅱ) for Injection by RP-HPLC. Methods:Semmetry RP-C18 column (4.6 mm×150 mm,5 μm) was used. The mobile phases consisted of methanol and water (gradient elution),the flow rate was 1.5 ml/min and the detection wavelength was at 265 nm. Results:The method was proved to be linear when concentrations of vitamin A,K1,D2 and E were in the range of 0.144-0.216 μg,0.240-0.360 μg,8.000-12.000 ng,14… CONTINUE READING