Contamination level and location of recreational freshwater influence the ability to predict Escherichia coli concentration by qPCR targeting Bacteroides.

@article{Mulugeta2012ContaminationLA,
  title={Contamination level and location of recreational freshwater influence the ability to predict Escherichia coli concentration by qPCR targeting Bacteroides.},
  author={Surafel Mulugeta and Ryan Hindman and Adam M Olszewski and Kaitlyn Hoover and Kendall Greene and Matthew D. Lieberman and Steven A. Mauro},
  journal={Journal of environmental management},
  year={2012},
  volume={103},
  pages={95-101}
}
Fecal bacteria are common microbial contaminants in freshwater with the potential to cause human illness. Detection of these microbes have traditionally relied on microbial plating to enumerate colonies of fecal indicator bacteria (FIB) such as Escherichia coli (E. coli), which can take 24 h or longer to complete. Quantitative PCR (qPCR) is a rapid and sensitive method for detection of FIB in recreational water that could compliment or potentially substitute for microbial plating. In this study… CONTINUE READING

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