Constructs for insertional mutagenesis, transcriptional signal localization and gene regulation studies in root nodule and other bacteria.

  title={Constructs for insertional mutagenesis, transcriptional signal localization and gene regulation studies in root nodule and other bacteria.},
  author={Wayne G Reeve and Ravi P. Tiwari and Penelope S. Worsley and Michael J. Dilworth and Andrew R. Glenn and John Gregory Howieson},
  volume={145 ( Pt 6)},
Cassettes have been developed that contain an antibiotic resistance marker with and without a promoterless gusA reporter gene. The nptII (encoding kanamycin resistance) or aacCI (encoding gentamicin resistance) genes were equipped with the tac promoter (Ptac) and the trpA terminator (TtrpA) and then cloned between NotI sites to construct the CAS-Nm (Ptac-nptII-TtrpA) and CAS-Gm (Ptac/PaacCI-aacCI-TtrpA) cassettes. The markers were also cloned downstream to a modified promoterless Escherichia… 

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