Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZ alpha and pUC18 or pUC19 multiple cloning sites.

@article{Jobling1990ConstructionOV,
  title={Construction of vectors with the p15a replicon, kanamycin resistance, inducible lacZ alpha and pUC18 or pUC19 multiple cloning sites.},
  author={Michael G. Jobling and RandallK. Holmes},
  journal={Nucleic acids research},
  year={1990},
  volume={18 17},
  pages={
          5315-6
        }
}
Complementation analysis is a powerful technique in the characterization of genes and their products. When studying cloned genes the analysis is facilitated if compatible vectors are available into which restriction fragments can be cloned. Most multicopy cloning vectors currently in use are derivatives of pBR322 (1), with ampicillin (Ap) resistance markers and the ColEl origin of replication. Vectors such as pUC18, pUC19 (2) and the pBluescript vectors (e.g. pKS-) (3) also contain a multiple… CONTINUE READING

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Greene.P.J., Betrach,M.C, Heyneker.H.L. and Boyer.H.W

  • Bolivar.F, R. L. Rodriguez
  • Gene
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