Construction of up-and-down temperature-jump apparatus.


A fast up-and-down temperature-jump apparatus whose dead time is about 60 ms was constructed. The principle of the method is to let the sample solution flow to the observation cell through a capillary in a heat-exchange chamber. Bubbling and cavitation effects in the observation cell at large up or down temperature jumps were eliminated by application of a nitrogen gas pressure of 2-5 bar. The down-temperature-jump method is especially effective for measuring temperature-induced conformational transitions of biopolymers and their assemblies.

Cite this paper

@article{Nakatani1985ConstructionOU, title={Construction of up-and-down temperature-jump apparatus.}, author={Hiroshi Nakatani}, journal={Analytical biochemistry}, year={1985}, volume={149 1}, pages={87-90} }