Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells.

@article{Hirano2008ConstructionOT,
  title={Construction of time-lapse scanning electrochemical microscopy with temperature control and its application to evaluate the preservation effects of antifreeze proteins on living cells.},
  author={Yu Hirano and Yoshiyuki Nishimiya and Keiko Kowata and Fumio Mizutani and Sakae Tsuda and Yasuo Komatsu},
  journal={Analytical chemistry},
  year={2008},
  volume={80 23},
  pages={9349-54}
}
Antifreeze proteins (AFPs) can protect cells from hypothermic damage; however, their mechanism of action remains unclear. Scanning electrochemical microscopy (SECM) can evaluate the size and activities of cells, although long-term continuous monitoring has been unsuccessful. We constructed a novel, fully automated, time-lapse SECM system and investigated the cell preservation effect of AFPs by analyzing single cellular topography at low temperatures. From the SECM measurements, mammalian cells… CONTINUE READING