Construction of the Coding Sequence of the Transcription Variant 2 of the Human Renalase Gene and Its Expression in the Prokaryotic System

@article{Fedchenko2013ConstructionOT,
  title={Construction of the Coding Sequence of the Transcription Variant 2 of the Human Renalase Gene and Its Expression in the Prokaryotic System},
  author={V. Fedchenko and A. Kaloshin and Lyudmila M. Mezhevikina and O. Buneeva and A. Medvedev},
  journal={International Journal of Molecular Sciences},
  year={2013},
  volume={14},
  pages={12764 - 12779}
}
Renalase is a recently discovered protein, involved in regulation of blood pressure in humans and animals. Although several splice variants of human renalase mRNA transcripts have been recognized, only one protein product, hRenalase1, has been found so far. In this study, we have used polymerase chain reaction (PCR)-based amplification of individual exons of the renalase gene and their joining for construction of full-length hRenalase2 coding sequence followed by expression of hRenalase2 as a… Expand
The catalytic function of renalase: A decade of phantoms.
  • G. Moran
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 2016
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