Construction of targeted plasmid vector pcDNA3.1-Egr.1p-p16 and its expression in pancreatic cancer JF305 cells induced by radiation in vitro.

@article{Ma2007ConstructionOT,
  title={Construction of targeted plasmid vector pcDNA3.1-Egr.1p-p16 and its expression in pancreatic cancer JF305 cells induced by radiation in vitro.},
  author={Hong-bing Ma and Xijng Wang and Zheng-li Di and H. Xia and Z. Li and J. Liu and Jie Ma and Hua-Fen Kang and Cong-mei Wu and Ming-hua Bai},
  journal={World journal of gastroenterology},
  year={2007},
  volume={13 31},
  pages={
          4214-8
        }
}
AIM To construct pcDNA3.1-Egr.1p-p16 recombinant plasmid and investigate the expression of p16 in pancreatic cancer JF305 cells induced by radiation and the feasibility of gene radiotherapy for pancreatic carcinoma. METHODS Human p16 cDNA was ligated to the downstream of Egr-1 promotor to construct pcDNA3.1-Egr.1p-p16 plasmid by restriction enzyme digested. The recombined plasmids were transfected into pancreatic cancer JF305 cells with lipofectamine. p16 mRNA level was detected by RT-PCR… Expand
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