Construction of stable, single-copy luciferase gene fusions in Escherichia coli

@article{Guzzo1991ConstructionOS,
  title={Construction of stable, single-copy luciferase gene fusions in Escherichia coli},
  author={Angelina Guzzo and Michael S. Dubow},
  journal={Archives of Microbiology},
  year={1991},
  volume={156},
  pages={444-448}
}
A ColE1-based plasmid for transcriptional gene fusions was constructed that contains both the promoterless luxAB genes of Vibrio harveyi and a tet marker within the inverted repeats of a left end-truncated Tn5 element. Introduction of this plasmid into an Escherichia coli strain containing a plasmid (pTF421) that overproduces ColE1 RNA1 (and thus inhibits replication of the ColE1 plasmid) allowed selection for cells that had a single copy of the luxAB operon transposed into the chromosome… CONTINUE READING