Construction of small RNA cDNA libraries for deep sequencing.

  title={Construction of small RNA cDNA libraries for deep sequencing.},
  author={Molly F Thomas and K Mark Ansel},
  journal={Methods in molecular biology},
Since the phenomenon of small RNA-mediated gene silencing was first described over 15 years ago (Lee et al. Cell 75:843-854, 1993; Wightman et al. Cell 75:855-862, 1993), it has become evident that a variety of endogenous small RNAs play an important role in establishing and maintaining cell lineages. MicroRNAs (miRNAs), in particular, have been shown to exert regulatory control over the development and function of the many specialized cells that comprise the mammalian immune system (Baltimore… CONTINUE READING


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