Construction of retroviral vector of p(125FAK) specific ribozyme genes and its effects on BGC-823 cells.

Abstract

AIM To construct the retroviral vector of p(125FAK) specific ribozyme genes and to explore the feasibility of ribozyme in BGC-823 gene therapy in vitro. METHODS A hammerhead ribozyme DNA targeting p(125FAK) mRNA from nt 1010 to nt 1032 was synthesized and recombined into the retroviral vector pLXSN forming pLRZXSN recon. Using the lipofectin-mediated DNA… (More)

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@article{Guan2006ConstructionOR, title={Construction of retroviral vector of p(125FAK) specific ribozyme genes and its effects on BGC-823 cells.}, author={Guo-xian Guan and Hong-Xing Jian and Dong-Yin Lei and Hui-shan Lu and Xiang-fu Zhang}, journal={World journal of gastroenterology}, year={2006}, volume={12 5}, pages={686-90} }