Construction of prophylactic human papillomavirus type 16 L1 capsid protein vaccine delivered by live attenuated Shigella flexneri strain sh42.

@article{Yang2005ConstructionOP,
  title={Construction of prophylactic human papillomavirus type 16 L1 capsid protein vaccine delivered by live attenuated Shigella flexneri strain sh42.},
  author={Xiao-Feng Yang and Xin-Zhong Qu and Kai Wang and Jin E. Zheng and L{\"u}-Sheng Si and Xiao-ping Dong and Yi-Li Wang},
  journal={Acta biochimica et biophysica Sinica},
  year={2005},
  volume={37 11},
  pages={743-50}
}
To express human papillomavirus (HPV) L1 capsid protein in the recombinant strain of Shigella and study the potential of a live attenuated Shigella-based HPV prophylactic vaccine in preventing HPV infection, the icsA/virG fragment of Shigella-based prokaryotic expression plasmid pHS3199 was constructed. HPV type 16 L1 (HPV16L1) gene was inserted into plasmid pHS3199 to form the pHS3199-HPV16L1 construct, and pHS3199-HPV16L1 was electroporated into a live attenuated Shigella strain sh42. Western… CONTINUE READING