Construction of promoter-probe shuttle vectors forEscherichia coli and corynebacteria on the basis of promoterless α-amylase gene

@article{Ugorcakova2000ConstructionOP,
  title={Construction of promoter-probe shuttle vectors forEscherichia coli and corynebacteria on the basis of promoterless α-amylase gene},
  author={Jana Ugorcakova and Gabriela Bukovsk{\'a} and Jozef Timko},
  journal={Folia Microbiologica},
  year={2000},
  volume={45},
  pages={114-120}
}
We constructed new promoter-probe vectors forE. coli and corynebacteria based on the promoterless α-amylase gene originating fromBacillus subtilis. Vectors pJUPAE1 and pJUPAE2 are suitable for isolation of transcriptionally active fragments from plasmids, phages or genomic DNA α-Amylase activity can be easily visually detected on agar plates containing a chromogenic substrate, or by direct measurement of α-amylase activity. 

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References

Publications referenced by this paper.
Showing 1-10 of 18 references

Construction of new cloning vectors for Brewbactermm lactofermentum

J. F. MARTIN, J. A. GIL
FEMS Mtcrobtol. LeII 137, • 1996

Multiple c ~ ihctors in Brevtbactertum lactofermentum : characterization of stgA and sigB

T. MARCOSA., M. MAI.UMBRE-S
J . Bacteriol . • 1996

Promoters from Coo ' nebactertum glutamtcum : cloning , molecular analysis and search for consensus motif

J. PATEK
Microbtology • 1996

[.F.: Multiple c~ ihctors in Brevtbactertum lactofermentum: characterization of stgA and sigB J.Bacteriol

MARTIN
1996

Characterization of bacteriophage BFK 20 from Bre vtbactermm flavum

M. IgOVA, E. BALOGtIOVA
1991

Construction and characterization promoter-probe vectors for corynebacteria using the kanamycin-resiscance reporter gone

R. F. CADt'NAS, J. A. MARTIN J.F.. GIL
Gene 98. I • 1991

Transformation of amino acid-producing brevibacteria with recombinant plasmids carrying the co-amylase gene

. 3ARAK, J. TIMKO
1991

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