Construction of prokaryotic expression vector of horseradish peroxidase gene

@inproceedings{Xiongxiong2011ConstructionOP,
  title={Construction of prokaryotic expression vector of horseradish peroxidase gene},
  author={Liu Xiong-xiong},
  year={2011}
}
The Horseradish Peroxidase C2 gene(hrpC2)was cloned from Armoracia rusticana(Horseradish)by means of reverse transcription. And the hrp was cloned into pPICZα-A vector successfully,after the hrpC2 and pPICZα-A plasmid vector was digested separately using EcoRI and XbaI. Then,the constructed pPICZα-A-hrpC2 plasmid vector was transformed into E.coli TOP10,and the positive recombinant clones were selected and checked. The result of sequence analyzing also indicted that the pPICZα-A-hrpC2 was… CONTINUE READING