Construction of primary and subtracted cDNA libraries from early embryos.

@article{Rothstein1993ConstructionOP,
  title={Construction of primary and subtracted cDNA libraries from early embryos.},
  author={Jay L. Rothstein and D. Randolph Johnson and Joel A. Jessee and J{\'o}zef Skowroński and Julie A Deloia and Davor Solter and Barbara B Knowles},
  journal={Methods in enzymology},
  year={1993},
  volume={225},
  pages={
          587-610
        }
}
By modifying current cDNA cloning and electroporation methods, large and representative murine cDNA libraries were synthesized from 10 to 100 ng mRNA isolated from unfertilized egg and preimplantation mouse embryos. High cloning efficiency is essential for complete representation of genes expressed in egg and preimplantation embryos and for the isolation of stage-specific genes using subtractive hybridization. Because the mouse embryo contains no more than 50 pg of poly(A)+ mRNA at any stage of… CONTINUE READING
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