Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit.

@article{Fontana1995ConstructionON,
  title={Construction of nontoxic derivatives of cholera toxin and characterization of the immunological response against the A subunit.},
  author={Maria Rita Fontana and Roberto Manetti and Valentina Giannelli and Claudia Magagnoli and Antonio Marchini and Roberto Olivieri and Mario Domenighini and Rino Rappuoli and Mariagrazia Pizza},
  journal={Infection and immunity},
  year={1995},
  volume={63 6},
  pages={2356-60}
}
Using computer modelling, we have identified some of the residues of the A subunit of cholera toxin (CT) and heat-labile toxin that are involved in NAD binding, catalysis, and toxicity. Here we describe the site-directed mutagenesis of the CT gene and the construction of CT mutants. Nine mutations of the A subunit gene were generated. Six of them encoded proteins that were fully assembled in the AB5 structure and were nontoxic; these proteins were CT-D53 (Val-53-->Asp), CT-K63 (Ser-63-->Lys… CONTINUE READING