Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement.

Abstract

Five cassettes carrying uidA, encoding beta-glucuronidase, were made for the construction of insertion mutants with transcriptional fusions to uidA. Three uidA cassettes contain antibiotic-resistance genes, for chloramphenicol (Cm), for kanamycin (Km) and neomycin (Nm), or for streptomycin (Sm) and spectinomycin (Sp). Some cause polar insertions while… (More)

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Cite this paper

@article{Metcalf1993ConstructionON, title={Construction of new beta-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement.}, author={William W Metcalf and Barry L. Wanner}, journal={Gene}, year={1993}, volume={129 1}, pages={17-25} }