Construction of new T vectors for direct cloning of PCR products.

Abstract

More than half of the products of PCR contain an extra A residue at the 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without modification, T vectors, which have a single overhanging T residue at the 3' end, have been developed. In the present study, we constructed new T vectors which can be prepared in the laboratory by simple digestion with the restriction enzymes AspEI or Eam1 105I.

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@article{Ichihara1993ConstructionON, title={Construction of new T vectors for direct cloning of PCR products.}, author={Yoshitatsu Ichihara and Yoshikazu Kurosawa}, journal={Gene}, year={1993}, volume={130 1}, pages={153-4} }