Construction of lentivirus-based inhibitor of hsa-microRNA-338-3p with specific secondary structure


Aim:To construct a lentivirus-based inhibitor with specific secondary structure that could exert long-term suppression on microRNA-338-3p (miR-338-3p), thus elucidating its molecular function in colorectal carcinoma cells.Methods:The miR-338-3p inhibitor sequence was synthesized and inserted into pLV-THM plasmid. HEK-293T cells were co-transfected with the… (More)
DOI: 10.1038/aps.2012.172


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