Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example.

@article{Huang2008ConstructionOI,
  title={Construction of infectious clones of double-stranded DNA viruses of plants using citrus yellow mosaic virus as an example.},
  author={Qi Hong Huang and John S Hartung},
  journal={Methods in molecular biology},
  year={2008},
  volume={451},
  pages={525-33}
}
Double-stranded DNA (dsDNA) viruses of plants are believed to be plant pararetroviruses. Their genome is replicated by reverse transcription of a larger than unit-length terminally redundant RNA transcript of the viral genomic DNA using the virus-encoded replicase. In order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. The clone can then be transferred by Agrobacterium-assisted inoculation… CONTINUE READING
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