Construction of improved bacteriophage phi 105 vectors for cloning by transfection in Bacillus subtilis.

@article{Jones1987ConstructionOI,
  title={Construction of improved bacteriophage phi 105 vectors for cloning by transfection in Bacillus subtilis.},
  author={David Jones and Jeff Errington},
  journal={Journal of general microbiology},
  year={1987},
  volume={133 3},
  pages={483-92}
}
A series of improved phage vectors have been constructed, based on Bacillus subtilis bacteriophage phi 105, which can be used to clone genes in B. subtilis by direct transfection of protoplasts. The new vectors, designated phi 105J23, phi 105J24, phi 105J27 and phi 105J28, show frequencies of plaque formation that are equal to those of wild-type phi 105. This represents at least a 10-fold improvement over phi 105J9, the vector used in previous cloning experiments. Two of the new vectors phi… CONTINUE READING