# Construction of hybrid viruses containing SV40 and λ phage DNA segments and their propagation in cultured monkey cells

@article{Goff1976ConstructionOH,
title={Construction of hybrid viruses containing SV40 and $\lambda$ phage DNA segments and their propagation in cultured monkey cells},
author={Stephen P. Goff and Paul W. Berg},
journal={Cell},
year={1976},
volume={9},
pages={695-705}
}
• Published 1 December 1976
• Biology
• Cell
126 Citations
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• 1981
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• Biology
Molecular and cellular biology
• 1984
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## References

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Journal of molecular biology
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Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments
• Biology, Chemistry
Nature
• 1974
Fragments of DNA from derivatives of phage λ having either one or two targets for R.EcoRI have been joined in new combinations to give biologically active phage genomes. These include two classes of
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The deoxyribonucleic acid exonuclease induced by bacteriophage λ has been obtained in crystalline and physically homogeneous form and may contain a trace (0.01%) of ex onuclease I.
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The stoichiometry, equilibrium, and kinetic properties of a terminal deoxynucleotidyltransferase isolated from calf thymus gland are described and Syntheses of polydeoxyadenylate, polyde oxygeninosinate, poly deoxycytidylates, and polydeoxythymidylate in greater than 80% yield are achieved under optimal conditions.
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Two specialized transducing phages are used as a source of complementary sequences from which to prepare pure lac operon duplex and the availability of a single pure promoter will facilitate many new experiments on genetic transcription.