Construction of hybrid viruses containing SV40 and λ phage DNA segments and their propagation in cultured monkey cells

@article{Goff1976ConstructionOH,
  title={Construction of hybrid viruses containing SV40 and $\lambda$ phage DNA segments and their propagation in cultured monkey cells},
  author={Stephen P. Goff and Paul W. Berg},
  journal={Cell},
  year={1976},
  volume={9},
  pages={695-705}
}
Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene
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TLDR
Evidence that a cloned mouse histone H4 gene contains all the information required for the generation of functional H4 mRNA is presented and the distribution of the H4 transcripts between the polysomal and postpolysomal fractions suggests that RNA initiated at the mouse promoter is more efficiently bound to polysomes than is the hybrid RNA initiatedat the SV40 promoter.
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TLDR
The structure of the messenger ribonucleic acids (mRNA's) formed during the expression of gpt and an unexpected feature of the nucleotide sequence in the gpt DNA segment are described and it is inferred that the bacterial enzyme can be translated from an initiator AUG that is 400 to 800 nucleotides from the 5' terminus of the mRNA and preceded by two to six AUG triplets.
BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli
TLDR
A novel expression vector that persists episomally in human cells that can be shuttled into bacteria is described, which includes sequences from BK virus, the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1.
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