Construction of hybrid viruses containing SV40 and λ phage DNA segments and their propagation in cultured monkey cells

  title={Construction of hybrid viruses containing SV40 and $\lambda$ phage DNA segments and their propagation in cultured monkey cells},
  author={Stephen P. Goff and Paul W. Berg},
Adaptation of a retrovirus as a eucaryotic vector transmitting the herpes simplex virus thymidine kinase gene
Great general applicability of retroviruses as eucaryotic vectors is suggested, as the chimeric MLV-tk virus showed single-hit kinetics in infections and behaved like known replication-defective retrovirus.
Molecular cloning of DNA
It is anticipated that bacterial host cells are not only suitable for amplifying DNA but also for the expression of useful functions which originate from other, preferably higher organisms.
Expression of cloned genes in eukaryotic cells using vector systems derived from viral replicons
The availability of chemical DNA sequencing techniques and the development by Sanger and his colleagues of the extraordinarily rapid dideoxy sequencing method which employs cloning in M13 mean that the determination of the complete nucleotide sequence of large segments of cloned DNA is now routine.
Expression of cloned genes in animal cells
The observation that carrier DNA greatly enhanced the transformation frequency led to successful attempts to transfer single copy eukaryotic genes using total genomic DNA and Wigler and colleagues then demonstrated that unlinked, non-selectable genes could be cotransformed when mixed in excess with selectable DNA.
A mouse histone H4 gene carried by an SV40 vector is accurately expressed in infected monkey cells.
Evidence that a cloned mouse histone H4 gene contains all the information required for the generation of functional H4 mRNA is presented and the distribution of the H4 transcripts between the polysomal and postpolysomal fractions suggests that RNA initiated at the mouse promoter is more efficiently bound to polysomes than is the hybrid RNA initiatedat the SV40 promoter.
Factors governing the expression of a bacterial gene in mammalian cells
The structure of the messenger ribonucleic acids (mRNA's) formed during the expression of gpt and an unexpected feature of the nucleotide sequence in the gpt DNA segment are described and it is inferred that the bacterial enzyme can be translated from an initiator AUG that is 400 to 800 nucleotides from the 5' terminus of the mRNA and preceded by two to six AUG triplets.
BK virus-plasmid expression vector that persists episomally in human cells and shuttles into Escherichia coli
A novel expression vector that persists episomally in human cells that can be shuttled into bacteria is described, which includes sequences from BK virus, the thymidine kinase (TK) gene of herpes simplex virus type 1, and plasmid pML-1.


Simian Virus 40 Deoxyribonucleic Acid Synthesis: the Viral Replicon
Temperature-shift experiments and analysis of SV40 viralDNA replication by gel electrophoresis have provided strong evidence that the ts gene product of the three mutants is directly required to initiate each new round of viral DNA replication but is not required to complete a cycle which has already begun.
Enzymatic end-to end joining of DNA molecules.
Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments
Fragments of DNA from derivatives of phage λ having either one or two targets for R.EcoRI have been joined in new combinations to give biologically active phage genomes. These include two classes of
An exonuclease induced by bacteriophage lambda. I. Preparation of the crystalline enzyme.
The deoxyribonucleic acid exonuclease induced by bacteriophage λ has been obtained in crystalline and physically homogeneous form and may contain a trace (0.01%) of ex onuclease I.
Deoxynucleotide-polymerizing enzymes of calf thymus gland. II. Properties of the terminal deoxynucleotidyltransferase.
The stoichiometry, equilibrium, and kinetic properties of a terminal deoxynucleotidyltransferase isolated from calf thymus gland are described and Syntheses of polydeoxyadenylate, polyde oxygeninosinate, poly deoxycytidylates, and polydeoxythymidylate in greater than 80% yield are achieved under optimal conditions.
Isolation of Pure lac Operon DNA*
Two specialized transducing phages are used as a source of complementary sequences from which to prepare pure lac operon duplex and the availability of a single pure promoter will facilitate many new experiments on genetic transcription.