Construction of human nonimmune library and selection of scFvs against IL-33.

Abstract

Interleukin (IL) 33 plays very important roles in inflammatory and allergic diseases. To select human single-chain Fv fragments (scFvs) against IL-33, a nonimmune phage library system was constructed. The full-length cDNA library was synthesized for amplification of the variable heavy chain (V(H)) and variable light chain (V(L)). By overlapping extension… (More)
DOI: 10.1007/s12010-012-9676-x

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