[Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells].

Abstract

AIM To construct human Egr-1 promoter luciferase reporter system and study its activity induced by ionizing radiation. METHODS Egr-1 promoter was obtained by human genomic PCR and cloned into pGL3-basic vector. After transfection of recombinant plasmid into human tumor cells, the Egr-1 promoter activity induced by ionizing radiation was detected by luciferase reporter assay. RESULTS The luciferase reporter system of Egr-1 promoter was successfully constructed. The activity of Egr-1 promoter was substantially increased after different doses of IR and reached to the peak at the time point of 48 h after IR. CONCLUSION The Egr-1 promoter was constructed in this study showed IR inducible activity in tumor cells, laying foundation for the research of radiation. mediated gene therapy.

Cite this paper

@article{Xu2009ConstructionOH, title={[Construction of human Egr-1 promoter and its response to ionizing radiation in tumor cells].}, author={Xiaojie Xu and Li-hua Ding and Ling-xue Wang and Xi Qin and Long Cheng and Kai Jiang and Qi-nong Ye}, journal={Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology}, year={2009}, volume={25 11}, pages={973-5} }