Construction of full-length cDNA clones of lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants

@article{Yang1998ConstructionOF,
  title={Construction of full-length cDNA clones of lettuce mosaic virus (LMV) and the effects of intron-insertion on their viability in Escherichia coli and on their infectivity to plants},
  author={Shu Jun Yang and Fr{\'e}d{\'e}ric Revers and S Yogesh Souche and Herv{\'e} Lot and Olivier le Gall and Thierry Candresse and Jean Dunez},
  journal={Archives of Virology},
  year={1998},
  volume={143},
  pages={2443-2451}
}
A full length cDNA copy of the genomic RNA of lettuce mosaic virus (LMV) was constructed under the control of an enhanced CaMV 35S promoter and of the NOS terminator. This construct was found infectious when inoculated to lettuce plants. The intron II of the bean nitrite reductase gene was engineered into the LMV FL cDNA in order to relieve possible deleterious effects of viral sequences to Escherichia coli cells and to evaluate the effects of the presence of the intron on the FL cDNA… CONTINUE READING

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Intron insertion facilitates amplification of cloned virus cDNA in Escherichia coli while biological activity is reestablished after transcription in vivo.

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Highly Influenced

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