Construction of expression vectors based on the rice actin 1 (Act1) 5′ region for use in monocot transformation

  title={Construction of expression vectors based on the rice actin 1 (Act1) 5′ region for use in monocot transformation},
  author={David McElroy and Alan D. Blowers and Barnab{\'a}s Jenes and Ray Yen-Hui Wu},
  journal={Molecular and General Genetics MGG},
It has been previously reported that the 5′ region of the rice actin 1 gene (Act1) promoted high-level expression of a β-glucuronidase reporter gene (Gus) in transformed rice cells. In this paper we describe the construction of Act1-based expression vectors for use in monocot transformation. As part of the development of these vectors, we have evaluated the influence of the Act1 first intron, the Act1-Gus junction-encoded N-terminal amino acids, and the sequence context surrounding the Act1 and… CONTINUE READING
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