Construction of expression systems for Escherichia coli asparaginase II and two-step purification of the recombinant enzyme from periplasmic extracts.

Abstract

Isoenzyme II of Escherichia coli L-asparaginase (L-asparagine amidohydrolase, EC 3.5.1.1) is among the few enzymes of major therapeutic importance, being used in the treatment of acute lymphoblastic leukemia. We have constructed several inducible expression systems that overproduce asparaginase II from recombinant plasmids. The most efficient of these… (More)

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@article{Harms1991ConstructionOE, title={Construction of expression systems for Escherichia coli asparaginase II and two-step purification of the recombinant enzyme from periplasmic extracts.}, author={Erik Harms and Anja Wehner and Maggy Jennings and Katherine Pugh and Ifor R. Beacham and Klaus H Roehm}, journal={Protein expression and purification}, year={1991}, volume={2 2-3}, pages={144-50} }