Construction of epidermal growth factor fusion protein with cell adhesive activity.

@article{Elloumi2006ConstructionOE,
  title={Construction of epidermal growth factor fusion protein with cell adhesive activity.},
  author={Imen Elloumi and Rie Kobayashi and Hisakage Funabashi and Masayasu Mie and Eiry Kobatake},
  journal={Biomaterials},
  year={2006},
  volume={27 18},
  pages={3451-8}
}
A novel protein for controlling cellular functions was constructed by combining functional units of various proteins. The Arg-Gly-Asp (RGD) sequence functioning as a cell adhesive function, an epidermal growth factor (EGF) as a cell growth function, and a hydrophobic sequence (E12) as an efficient assembling function, were combined and incorporated into one molecule. The fusion protein, designated ERE-EGF, was produced in Escherichia coli and purified with affinity chromatography using a His… CONTINUE READING