Construction of derivatives of the F plasmid pOX-tra715: characterization of traY and traD mutants that can be complemented in trans.

@article{Maneewannakul1996ConstructionOD,
  title={Construction of derivatives of the F plasmid pOX-tra715: characterization of traY and traD mutants that can be complemented in trans.},
  author={K Maneewannakul and P Kathir and Seema Endley and Deanna Moore and Jan Manchak and Laura S. Frost and Karin Ippen-Ihler},
  journal={Molecular microbiology},
  year={1996},
  volume={22 2},
  pages={197-205}
}
A derivative of the F plasmid, pOX38-tra715, expresses the entire F tra operon from a foreign promoter (PT7) derived from phage T7. A series of plasmids related to pOX38-tra715 were constructed which carry either deletion mutations or point mutations in traY. When the PT7 promoter was induced, these plasmids expressed the F pilus but were transfer deficient unless TraY was supplied In trans from compatible plasmids. Insertion of a kanamycin-resistance cassette in the traY gene of the pOX38… CONTINUE READING

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