Construction of chimeric thymuses in the mouse fetus by in utero surgery.

  title={Construction of chimeric thymuses in the mouse fetus by in utero surgery.},
  author={Josselyne Sala{\"u}n and Frances M. B. Calman and Monique Coltey and Nicole M. Le Douarin},
  journal={European journal of immunology},
  volume={16 5},
Normal thymuses develop in the coelomic cavity of mice following grafts carried out in utero into embryonic day 16 to 17 fetuses of the third branchial arch region removed from 10-day embryos. The recipients were killed at various times (up to 29 days after birth) and the thymic lobes which developed from the graft were recovered. Microscopical examination and immunocytochemistry revealed that these thymuses developed normally whether the grafts involved syngeneic or allogeneic (between BALB/c… CONTINUE READING

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