Construction of chimeric cyclodextrin glucanotransferases from Bacillus circulans A11 and Paenibacillus macerans IAM1243 and analysis of their product specificity.

@article{Rimphanitchayakit2005ConstructionOC,
  title={Construction of chimeric cyclodextrin glucanotransferases from Bacillus circulans A11 and Paenibacillus macerans IAM1243 and analysis of their product specificity.},
  author={Vichien Rimphanitchayakit and Takashi Tonozuka and Yoshiyuki Sakano},
  journal={Carbohydrate research},
  year={2005},
  volume={340 14},
  pages={2279-89}
}
Three DNA fragments of 7919 base pairs containing genes for beta-cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19), an iron III dicitrate transport protein-like protein and a partial coding sequence for putative ferrichrome ABC transporter from Bacillus circulans A11 were cloned and sequenced (GenBank Accession AF302787). The DNA sequence contained a CGTase open reading frame of 2139 base pairs, which encoded a polypeptide of 713 amino acid residues. The signal peptide constituted the N… CONTINUE READING

Similar Papers

Loading similar papers…