Construction of broad-host-range vectors for the selection of divergent promoters.

@article{Ronald1990ConstructionOB,
  title={Construction of broad-host-range vectors for the selection of divergent promoters.},
  author={S L Ronald and Andrew M Kropinski and Mark A. Farinha},
  journal={Gene},
  year={1990},
  volume={90 1},
  pages={145-8}
}
A series of promoter-probe plasmid vectors has been constructed which allows for the selection of DNA sequences containing divergent control elements. Each vector contains a pair of promoterless genes [encoding beta-galactosidase (lacZ), alkaline phosphatase (phoA), and bacterial luciferase (luxAB)] arranged in an antiparallel fashion and separated by a large intervening multiple cloning site. The vectors permit direct detection of promoter activity on indicator plates after transformation… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Similar Papers

Loading similar papers…