Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production.

@article{Hart1992ConstructionOA,
  title={Construction of an insertional-inactivation cloning vector for Escherichia coli using a Rhodococcus gene for indigo production.},
  author={S Hart and David R. Woods},
  journal={Journal of general microbiology},
  year={1992},
  volume={138 1},
  pages={205-9}
}
pSLH8, an insertional-inactivation cloning vector for Escherichia coli has been constructed by inserting a pigment gene (probably encoding an indole dioxygenase) from Rhodococcus sp. ATCC 21145 into pUC18. Wild-type E. coli colonies containing pSLH8 produce insoluble indigo and turn dark blue on unsupplemented LB agar. Insertion of DNA fragments into the… CONTINUE READING