Construction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly.

@article{Blawid2015ConstructionOA,
  title={Construction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly.},
  author={R. Blawid and T. Nagata},
  journal={Journal of virological methods},
  year={2015},
  volume={222},
  pages={
          11-5
        }
}
The construction of full-length infectious clones of RNA viruses is often laborious due to the many cloning steps required and the DNA exclusion within the plasmid during Escherichia coli transformation. We demonstrate single-step cloning procedure of an infectious cDNA of the tomato blistering mosaic virus (ToBMV) using Gibson Assembly (GA), which drastically reduces the number of cloning steps. By agro-inoculation with the construct obtained by this procedure, ToBMV was recovered six days… Expand
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