Construction of an h+Sstrain of Schizosaccharomyces pombe

@article{Heim2004ConstructionOA,
  title={Construction of an h+Sstrain of Schizosaccharomyces pombe},
  author={Lutz Heim},
  journal={Current Genetics},
  year={2004},
  volume={17},
  pages={13-19}
}
  • L. Heim
  • Published 2004
  • Biology, Materials Science
  • Current Genetics
SummaryBy spontaneous in vivo integration of a mat2:1° plasmid, containing a Plus (P) cassette, into an h-LMT region of Schizosaccharomyces pombe an h+ strain was obtained which neither mutates to h- nor to h90. Southern blotting showed that it possesses the same mating-type (MT) configuration as h-S except that P information resides in both cassettes. Therefore the strain was called h+S. By crossing h+S with the h- strain LK42 of Engelke et al. (1987) it was possible to obtain h- recombinants… 

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