• Corpus ID: 39326132

Construction of an h+S strain of Schizosaccharomyces pombe.

  title={Construction of an h+S strain of Schizosaccharomyces pombe.},
  author={Lutz Heim},
  journal={Current genetics},
  volume={17 1},
  • L. Heim
  • Published 1990
  • Biology
  • Current genetics
By spontaneous in vivo integration of a mat2:1 degrees plasmid, containing a Plus (P) cassette, into an h-L MT region of Schizosaccharomyces pombe an h+ strain was obtained which neither mutates to h- nor to h90. Southern blotting showed that it possesses the same mating-type (MT) configuration as h-S except that P information resides in both cassettes. Therefore the strain was called h+S. By crossing h+S with the h- strain LK42 of Engelke et al. (1987) it was possible to obtain h- recombinants… 
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