Construction of an engineered strain capable of degrading two isomeric nitrophenols via a sacB- and gfp-based markerless integration system

@article{Hu2014ConstructionOA,
  title={Construction of an engineered strain capable of degrading two isomeric nitrophenols via a sacB- and gfp-based markerless integration system},
  author={Fan Hu and Xuan Jiang and Junjie Zhang and Ning-Yi Zhou},
  journal={Applied Microbiology and Biotechnology},
  year={2014},
  volume={98},
  pages={4749-4756}
}
In this study, a gfp-based novel markerless allelic exchange integration system was developed. By employing gfp gene and sacB gene as counter-selectable markers, an ortho-nitrophenol degradation operon (onpABC gene cluster) was successfully inserted into the chromosome of meta-nitrophenol utilizer Cupriavidus necator JMP134. Through two rounds of recombination, the engineered strain (strain JMP134-ONP) was directly selected from the plate by fluorescence screening and has the ability to degrade… CONTINUE READING