Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector.

@article{Donnenberg1991ConstructionOA,
  title={Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector.},
  author={Michael S. Donnenberg and J. Kaper},
  journal={Infection and immunity},
  year={1991},
  volume={59 12},
  pages={4310-7}
}
The ability to attach to epithelial cells, efface the microvillus surface, and disrupt the underlying cytoskeleton is characteristic of enteropathogenic Escherichia coli (EPEC). Recently, eae, a gene necessary for this phenomenon, was described (A. E. Jerse, J. Yu, B. D. Tall, and J. B. Kaper, Proc. Natl. Acad. Sci. USA 87:7839-7843, 1990). We report the use of a novel suicide vector containing the pir-dependent R6K replicon and the sacB gene of Bacillus subtilis to construct an eae deletion… CONTINUE READING
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Direct DNA sequencing of polymerase chain reaction products, p. 15.2.1-15.2.11

  • R. L. Dorit, 0. Ohara
  • 1990

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