Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol of Saccharomyces cerevisiae

@article{Matsuda2012ConstructionOA,
  title={Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol of Saccharomyces cerevisiae},
  author={Fumio Matsuda and Takashi Kondo and Kengo Ida and Hironori Tezuka and Jun Ishii and Akihiko Kondo},
  journal={Bioscience, Biotechnology, and Biochemistry},
  year={2012},
  volume={76},
  pages={2139 - 2141}
}
To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L). 
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Construction of Artificial Pathway for Isobutanol Production