Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol of Saccharomyces cerevisiae

  title={Construction of an Artificial Pathway for Isobutanol Biosynthesis in the Cytosol of Saccharomyces cerevisiae},
  author={Fumio Matsuda and Takashi Kondo and Kengo Ida and Hironori Tezuka and Jun Ishii and Akihiko Kondo},
  journal={Bioscience, Biotechnology, and Biochemistry},
  pages={2139 - 2141}
To increase isobutanol production in Saccharomyces cerevisiae, the valine biosynthetic pathway was activated by overexpression of the relevant enzymes in the mitochondria and the cytosol. Native mitochondrial enzymes were overepxressed in the cytosol by deleting the mitochondrial transit peptides. The metabolically engineered S. cerevisiae possessing the cytosolic pathway showed increased isobutanol production (63 ± 4 mg/L). 
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Increased isobutanol production in Saccharomyces cerevisiae by eliminating competing pathways and resolving cofactor imbalance
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Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae
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Heterologous expression of bacterial phosphoenol pyruvate carboxylase and Entner-Doudoroff pathway in Saccharomyces cerevisiae for improvement of isobutanol production.
Results showed that the ED pathway was successfully constructed in S. cerevisiae, even though activity of the pathway was too weak to improve isobutanol biosynthesis. Expand
Improving isobutanol titers in Saccharomyces cerevisiae with over-expressing NADPH-specific glucose-6-phosphate dehydrogenase (Zwf1)
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Genetically encoded biosensors for branched-chain amino acid metabolism to monitor mitochondrial and cytosolic production of isobutanol and isopentanol in yeast
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A global review of existing research for selected, engineered microorganisms designed to produce higher-order biofuels is offered in contrast with Crispr-Cas9 genome editing technology that will play an increasingly important role, which can be used to overcome the complex genetic metabolic background of microorganisms at more advanced levels. Expand
Improving isobutanol tolerance and titers through EMS mutagenesis in Saccharomyces cerevisiae.
EMS mutagenesis could be used to increase yeast tolerance toward isobutanol and enhancing isobUTanol production in S.cerevisiae, and reverse engineering tests showed that overexpression of CWP2 and SRP4039 could improve tolerance of S.CErevisae toward isOButanol. Expand
Bacterial production of isobutanol without expensive reagents
A simple and efficient method for isobutanol production in Escherichia coli is described, and results indicate that the developed strain is potentially useful for industrial isobUTanol production. Expand


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Identification and Functional Expression of the Mitochondrial Pyruvate Carrier
The existence of a specific mitochondrial pyruvate carrier (MPC) has been anticipated, but its molecular identity remained unknown and it is reported that MPC is a heterocomplex formed by two members of a family of previously uncharacterized membrane proteins that are conserved from yeast to mammals. Expand
Construction of Artificial Pathway for Isobutanol Production