Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs.

@article{Yerle1998ConstructionOA,
  title={Construction of a whole-genome radiation hybrid panel for high-resolution gene mapping in pigs.},
  author={Martine Yerle and Philippe Pinton and Annie Robic and Abraham Alfonso and Y Palvadeau and Chantal Delcros and Rachel Hawken and Lee Alexander and Craig Beattie and Lawrence B. Schook and Denis Milan and Joel Gellin},
  journal={Cytogenetics and cell genetics},
  year={1998},
  volume={82 3-4},
  pages={182-8}
}
We have developed a panel of 152 whole-genome radiation hybrids by fusing irradiated diploid pig lymphocytes or fibroblasts with recipient hamster permanent cells. The number and size of the porcine chromosome fragments retained in each hybrid clone were checked by fluorescence in situ hybridization with a SINE probe or by primed in situ labeling (PRINS) with SINE-specific primers. A strategy based on the interspersed repetitive sequence polymerase chain reaction (IRS-PCR) was developed for… CONTINUE READING
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