Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

@article{Simon1988ConstructionOA,
  title={Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.},
  author={Dietrich Simon and Alain Chopin},
  journal={Biochimie},
  year={1988},
  volume={70 4},
  pages={559-66}
}
Cloning vector plasmids have been constructed on the basis of the broad host range plasmid pAM beta 1 and used for the cloning of a nisin resistance determinant in Streptococcus lactis. They incorporate several desirable features for gene cloning in S. lactis and other transformable Gram-positive bacteria. They carry an easily selectable erythromycin resistance marker, are present at low (6-9) or high (45-85) copy number in S. lactis and possess a convenient polyrestriction site sequence. A… CONTINUE READING

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