Construction of a tRNA-embedded-ribozyme trimming plasmid.


We have combined Cotten and Birnstiel's tRNA-embedded ribozymes and our 5'- and 3'-trimming system. Although the activity of the tRNA-embedded ribozyme was ca. 30% lower than those of naked ribozymes, since the stability of the former in bovine serum was higher than those of naked ribozymes, the tRNA-embedded ribozymes appear useful especially when the 5… (More)


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@article{Yuyama1992ConstructionOA, title={Construction of a tRNA-embedded-ribozyme trimming plasmid.}, author={Nana Yuyama and Jun Ohkawa and Y. Inokuchi and Mitsuyuki Shirai and Atsuhisa Sato and Shin Nishikawa and Kazunari Taira}, journal={Biochemical and biophysical research communications}, year={1992}, volume={186 3}, pages={1271-9} }