Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements

@article{RogelGaillard1999ConstructionOA,
  title={Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements},
  author={Claire Rogel-Gaillard and Noelle Bourgeaux and Alain Billault and Marcel Vaiman and Patrick Chardon},
  journal={Cytogenetic and Genome Research},
  year={1999},
  volume={85},
  pages={205 - 211}
}
A porcine bacterial artificial chromosome (BAC) library was constructed using the pBeloBAC11 vector. It comprised 107,520 clones with an average insert size of 135 kb, representing an almost fivefold coverage of the swine haploid genome. Screening of the library allowed recovery of one to eight clones for 142 unique markers located all over the genome, while it failed for only one marker. About 4% chimeric clones were found. The library was also screened for the protease gene of type C porcine… Expand
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