Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant.

@article{Bertrand1984ConstructionOA,
  title={Construction of a single-copy promoter vector and its use in analysis of regulation of the transposon Tn10 tetracycline resistance determinant.},
  author={Kevin P. Bertrand and Kathleen Postle and Lewis V. Wray and W. S. Reznikoff},
  journal={Journal of bacteriology},
  year={1984},
  volume={158 3},
  pages={910-9}
}
The construction and characterization of a promoter expression vector, lambda RS205 , is described. lambda RS205 can be used for the in vitro construction of transcriptional (operon) fusions to the lacZ gene of Escherichia coli K-12. The level of beta-galactosidase activity in lysogens of lambda RS205 fusion phages provides a quantitative measure of promoter function under single-copy conditions. The regulation of the Tn10 tetracycline resistance gene ( tetA ) and the Tn10 tet repressor gene… CONTINUE READING

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