Construction of a shuttle vector for use in Francisella tularensis.

  title={Construction of a shuttle vector for use in Francisella tularensis.},
  author={A. Norqvist and Kerstin Kuoppa and Gunnar Sandstr{\"o}m},
  journal={FEMS immunology and medical microbiology},
  volume={13 3},
The characterisation of virulence factors of Francisella tularensis has been hampered by the lack of genetic system for the bacterium. In this study, a shuttle vector was constructed that can replicate autonomously in F. tularensis and Escherichia coli. To obtain this vector, the p15A replication origin of F. coli plasmid pACYC184 was introduced into a plasmid derivative of plasmid pFNL2OO, a plasmid which only can replicate in F. tularensis. The resulting shuttle vector, designated pKK2O2… CONTINUE READING

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