Construction of a pTOC-T vector using GST-ParE toxin for direct cloning and selection of PCR products

@article{Kim2004ConstructionOA,
  title={Construction of a pTOC-T vector using GST-ParE toxin for direct cloning and selection of PCR products},
  author={Han Geun Kim and Hye Sun Kim and H. Hwang and S. Chung and J. Lee and D. Chung},
  journal={Biotechnology Letters},
  year={2004},
  volume={26},
  pages={1659-1663}
}
Using linker insertion mutations, we determined the most stable region of the parE gene which encodes a toxic protein (ParE) that inhibits growth of Escherichia coli. The toxicity of ParE was sustained until a 144 bp linker was inserted into this region. We have developed a 3′ T-overhang vector based on these characteristics of the GST-ParE toxin, and named pTOC-T. Because pTOC-T uses a post-segregational killing system, all transformants grown up on the plates can be considered as recombinants… Expand
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References

SHOWING 1-10 OF 11 REFERENCES
Plasmid RK2 toxin protein ParE: purification and interaction with the ParD antitoxin protein.
Fluorescent protein vector for directional selection of PCR clones.
The parDE operon of the broad-host-range plasmid RK2 specifies growth inhibition associated with plasmid loss.
Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases.
  • J. Clark
  • Biology, Medicine
  • Nucleic acids research
  • 1988
T vectors with endoglucanase A (celA) gene for direct detection of PCR clones.
...
1
2
...