Construction of a new recombinant protein expression system in the basidiomycetous yeast Cryptococcus sp. strain S-2 and enhancement of the production of a cutinase-like enzyme

@article{Masaki2011ConstructionOA,
  title={Construction of a new recombinant protein expression system in the basidiomycetous yeast Cryptococcus sp. strain S-2 and enhancement of the production of a cutinase-like enzyme},
  author={Kazuo Masaki and Hiroaki Tsuchioka and Takuya Hirano and Miyoshi Kato and Hiroko Ikeda and Haruyuki Iefuji},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={93},
  pages={1627-1636}
}
Yeast host–vector systems have been very successful in expressing recombinant proteins. However, because there are some proteins that cannot be expressed with existing systems, there is a need for new yeast expression systems. Here we describe a new host–vector system based on the basidiomycetous yeast Cryptococcus sp. strain S-2 (S-2). Two advantages of S-2 are that it naturally produces some very useful enzymes, so it would be a good system for expressing multiple copies of some of its genes… CONTINUE READING

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