Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.

@article{Stanley1984ConstructionOA,
  title={Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.},
  author={Keith K. Stanley and J Paul Luzio},
  journal={The EMBO journal},
  year={1984},
  volume={3 6},
  pages={
          1429-34
        }
}
Construction of a family of bacterial expression vectors, pEX1-3, is described. These vectors are derived from a cro-lacZ gene fusion plasmid which expresses large quantities of fusion protein under the control of the PR promoter of bacteriophage lambda. A polylinker has been engineered into the 3' end of the lacZ gene in all three translational reading frames, and stop signals for transcription and translation inserted, so that any open reading frame DNA may be expressed as a hybrid beta… CONTINUE READING
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