Construction of a mutant of Escherichia coli that has deletions of both the penicillin-binding protein 5 and 6 genes.

Abstract

A mutant of Escherichia coli has been constructed with deletions of the genes encoding penicillin-binding protein 5 (dacA) and penicillin-binding protein 6 (dacC). The construction of this mutant establishes that the complete loss of the two most abundant species of penicillin-binding proteins can be tolerated by E. coli. Moreover, the double deletion… (More)

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